Use of a PNA probe to block DNA-mediated PCR product formation in prokaryotic RT-PCR

Use of a PNA probe to block DNA-mediated PCR product formation in prokaryotic RT-PCR

A novel method eliminating DNA-mediated PCR product formation in reverse transcription PCR (RT-PCR) amplification of specific RNA sequences is described. The method exploits the higher melting temperature values of peptide nucleic acid (PNA)/DNA duplexes compared with DNA/DNA duplexes by binding a s...

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Veröffentlicht in:BioTechniques. - 1993. - 42(2007), 5 vom: 01. Mai, Seite 609-10, 612-4
1. Verfasser: Bender, Mikkel (VerfasserIn)
Weitere Verfasser: Holben, William E, Sørensen, Søren J, Jacobsen, Carsten S
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Probes DNA, Bacterial Nucleic Acid Heteroduplexes Peptide Nucleic Acids DNA 9007-49-2