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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed25n0568.xml
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| 041 |
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|a eng
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| 100 |
1 |
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|a Bender, Mikkel
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Use of a PNA probe to block DNA-mediated PCR product formation in prokaryotic RT-PCR
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| 264 |
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1 |
|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 22.06.2007
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|a Date Revised 07.06.2018
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a A novel method eliminating DNA-mediated PCR product formation in reverse transcription PCR (RT-PCR) amplification of specific RNA sequences is described. The method exploits the higher melting temperature values of peptide nucleic acid (PNA)/DNA duplexes compared with DNA/DNA duplexes by binding a sequence-specific PNA probe to a genomic sequence immediately overlapping one of the PCR-primer attachment sites within the sequence of interest. Hybridization of the blocking probe precludes primer attachment to DNA without affecting attachment of the same primer to the reverse transcription-generated cDNA sequence. A four-step PCR cycle is used that allows the PNA probe to hybridize to the DNA strand at a higher temperature just prior to the primer annealing step
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a DNA Probes
|2 NLM
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| 650 |
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7 |
|a DNA, Bacterial
|2 NLM
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| 650 |
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7 |
|a Nucleic Acid Heteroduplexes
|2 NLM
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| 650 |
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7 |
|a Peptide Nucleic Acids
|2 NLM
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Holben, William E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sørensen, Søren J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jacobsen, Carsten S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 42(2007), 5 vom: 01. Mai, Seite 609-10, 612-4
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:42
|g year:2007
|g number:5
|g day:01
|g month:05
|g pages:609-10, 612-4
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