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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0567.xml
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|a (DE-627)NLM169995968
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|a (NLM)17469859
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Horton, Margaret R
|e verfasserin
|4 aut
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| 245 |
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|a Structure and dynamics of crystalline protein layers bound to supported lipid bilayers
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 11.07.2007
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|a Date Revised 20.11.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We study proteins at the surface of bilayer membranes using streptavidin and avidin bound to biotinylated lipids in a supported lipid bilayer (SLB) at the solid-liquid interface. Using X-ray reflectivity and simultaneous fluorescence microscopy, we characterize the structure and fluidity of protein layers with varied relative surface coverages of crystalline and noncrystalline protein. With continuous bleaching, we measure a 10-15% decrease in the fluidity of the SLB after the full protein layer is formed. We propose that this reduction in lipid mobility is due to a small fraction (0.04) of immobilized lipids bound to the protein layer that create obstacles to membrane diffusion. Our X-ray reflectivity data show a 40 A thick layer of protein, and we resolve an 8 A layer separating the protein layer from the bilayer. We suggest that the separation provided by this water layer allows the underlying lipid bilayer to retain its fluidity and stability
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fluorescent Dyes
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Macromolecular Substances
|2 NLM
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|a Proteins
|2 NLM
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|a Spin Labels
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Avidin
|2 NLM
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|a 1405-69-2
|2 NLM
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| 650 |
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|a Streptavidin
|2 NLM
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| 650 |
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|a 9013-20-1
|2 NLM
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| 700 |
1 |
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|a Reich, Christian
|e verfasserin
|4 aut
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|a Gast, Alice P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rädler, Joachim O
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nickel, Bert
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 11 vom: 22. Mai, Seite 6263-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:23
|g year:2007
|g number:11
|g day:22
|g month:05
|g pages:6263-9
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|h 6263-9
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