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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0566.xml
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|a (DE-627)NLM169792706
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|a (NLM)17447800
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Chifen, Anye N
|e verfasserin
|4 aut
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| 245 |
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|a Attachment and phospholipase A2-induced lysis of phospholipid bilayer vesicles to plasma-polymerized maleic anhydride/SiO2 multilayers
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 11.07.2007
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|a Date Revised 24.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydride films covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combined with surface plasmon resonance spectroscopy (SPR) was used to monitor the activation of plasma-deposited maleic anhydride (pp-MA) film with EDC/NHS and the subsequent coupling of lipid vesicles. The vesicles were formed from a mixture of phosphatidylcholine and phosphatidylethanolamine lipids, with a water-soluble fluorophore encapsulated within. Vesicle attachment was measured in real time on plasma films formed under different pulse conditions (plasma duty cycle). Optimum vesicle attachment was observed on the pp-MA films containing the highest density of maleic anhydride groups. Phospholipase A2 was used to lyse the surface-bound vesicles and to release the encapsulated fluorophore
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Coated Materials, Biocompatible
|2 NLM
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|a Fluorescent Dyes
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|a Lipid Bilayers
|2 NLM
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|a Maleic Anhydrides
|2 NLM
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|a Phosphatidylethanolamines
|2 NLM
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|a Gold
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|a 7440-57-5
|2 NLM
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|a Silicon Dioxide
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|a 7631-86-9
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|a Phospholipases A
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|a EC 3.1.1.32
|2 NLM
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|a Phospholipases A2
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|a EC 3.1.1.4
|2 NLM
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|a Dimyristoylphosphatidylcholine
|2 NLM
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| 650 |
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|a U86ZGC74V5
|2 NLM
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| 650 |
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|a 1,2-dimyristoylphosphatidylethanolamine
|2 NLM
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|a Z37OX1ASNK
|2 NLM
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| 700 |
1 |
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|a Förch, Renate
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Knoll, Wolfgang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cameron, Petra J
|e verfasserin
|4 aut
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| 700 |
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|a Khor, Hwei L
|e verfasserin
|4 aut
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|a Williams, Thomas L
|e verfasserin
|4 aut
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|a Jenkins, A Toby A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 11 vom: 22. Mai, Seite 6294-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:23
|g year:2007
|g number:11
|g day:22
|g month:05
|g pages:6294-8
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_721
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|a AR
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|d 23
|j 2007
|e 11
|b 22
|c 05
|h 6294-8
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