Attachment and phospholipase A2-induced lysis of phospholipid bilayer vesicles to plasma-polymerized maleic anhydride/SiO2 multilayers

This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydride films covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combined with surface plasmon resonance spectroscopy...

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Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 11 vom: 22. Mai, Seite 6294-8
1. Verfasser: Chifen, Anye N (VerfasserIn)
Weitere Verfasser: Förch, Renate, Knoll, Wolfgang, Cameron, Petra J, Khor, Hwei L, Williams, Thomas L, Jenkins, A Toby A
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Coated Materials, Biocompatible Fluorescent Dyes Lipid Bilayers Maleic Anhydrides Phosphatidylethanolamines Gold 7440-57-5 Silicon Dioxide mehr... 7631-86-9 Phospholipases A EC 3.1.1.32 Phospholipases A2 EC 3.1.1.4 Dimyristoylphosphatidylcholine U86ZGC74V5 1,2-dimyristoylphosphatidylethanolamine Z37OX1ASNK
Beschreibung
Zusammenfassung:This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydride films covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combined with surface plasmon resonance spectroscopy (SPR) was used to monitor the activation of plasma-deposited maleic anhydride (pp-MA) film with EDC/NHS and the subsequent coupling of lipid vesicles. The vesicles were formed from a mixture of phosphatidylcholine and phosphatidylethanolamine lipids, with a water-soluble fluorophore encapsulated within. Vesicle attachment was measured in real time on plasma films formed under different pulse conditions (plasma duty cycle). Optimum vesicle attachment was observed on the pp-MA films containing the highest density of maleic anhydride groups. Phospholipase A2 was used to lyse the surface-bound vesicles and to release the encapsulated fluorophore
Beschreibung:Date Completed 11.07.2007
Date Revised 24.11.2016
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827