Immunoblotting on HEp-2 cells increases the detection of antitopoisomerase 1 antibodies in patients with systemic sclerosis

Immunoblotting on HEp-2 cells increases the detection of antitopoisomerase 1 antibodies in patients with systemic sclerosis

In order to improve the detection of antitopoisomerase 1 antibodies in a cohort of 111 systemic sclerosis patients, we have performed immunoblots on protein extracts of HEp-2 cells. Using indirect immunofluorescence and ELISA, 27 patients (24.3%) had antitopoisomerase 1 antibodies, 32 (28.8%) had an...

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Publié dans:Clinical immunology (Orlando, Fla.). - 1999. - 123(2007), 1 vom: 30. Apr., Seite 82-8
Auteur principal: Tamby, Mathieu C (Auteur)
Autres auteurs: Servettaz, Amélie, Guilpain, Philippe, Tamas, Nicolas, Berezné, Alice, Batteux, Frédéric, Reinbolt, Joseph, Guillevin, Loïc, Weill, Bernard, Mouthon, Luc
Format: Article
Langue:English
Publié: 2007
Accès à la collection:Clinical immunology (Orlando, Fla.)
Sujets:Journal Article Research Support, Non-U.S. Gov't Antibodies, Antinuclear Autoantibodies Autoantigens Immunoglobulin G DNA Topoisomerases, Type I EC 5.99.1.2