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231223s2006 xx ||||| 00| ||eng c |
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|a (NLM)17107015
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Gujraty, Kunal V
|e verfasserin
|4 aut
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| 245 |
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|a Thiol-mediated anchoring of ligands to self-assembled monolayers for studies of biospecific interactions
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 13.03.2007
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|a Date Revised 24.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a We report a method to immobilize thiol-containing ligands onto self-assembled monolayers (SAMs) of alkanethiolates presenting chloracetylated hexa(ethylene glycol) groups. The chloroacetyl groups react with thiols under mild basic conditions, enabling the stable immobilization of biologically active ligands in a well-defined orientation. These SAMs on gold are well suited for studies of biospecific interactions of immobilized ligands with proteins and cells. As a demonstration, we functionalized these SAMs with thiol-containing derivatives of biotin and benzene sulfonamide and observed the specific binding of neutravidin and carbonic anhydrase, respectively. We also used this method to generate mixed SAMs presenting the Arg-Gly-Asp (RGD) peptide sequence and demonstrated the integrin-mediated adhesion of fibroblast cells to these SAMs. This approach would allow the immobilization of proteins and other sensitive biomolecules and ligands for a wide variety of applications in biotechnology
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|a Journal Article
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|a Alkanes
|2 NLM
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|a Ligands
|2 NLM
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|a Oligopeptides
|2 NLM
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7 |
|a Proteins
|2 NLM
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|a Sulfhydryl Compounds
|2 NLM
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| 650 |
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|a Sulfonamides
|2 NLM
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| 650 |
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|a neutravidin
|2 NLM
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| 650 |
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|a Avidin
|2 NLM
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|a 1405-69-2
|2 NLM
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|a Biotin
|2 NLM
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|a 6SO6U10H04
|2 NLM
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|a arginyl-glycyl-aspartic acid
|2 NLM
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| 650 |
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|a 78VO7F77PN
|2 NLM
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|a Carbonic Anhydrases
|2 NLM
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| 650 |
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|a EC 4.2.1.1
|2 NLM
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| 650 |
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|a Benzene
|2 NLM
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| 650 |
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|a J64922108F
|2 NLM
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| 700 |
1 |
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|a Ashton, Randolph
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bethi, Sridhar R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kate, Sandesh
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Faulkner, Christopher J
|e verfasserin
|4 aut
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|a Jennings, G Kane
|e verfasserin
|4 aut
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|a Kane, Ravi S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 24 vom: 21. Nov., Seite 10157-62
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:22
|g year:2006
|g number:24
|g day:21
|g month:11
|g pages:10157-62
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