Simple one-week method to construct gene-targeting vectors application to production of human knockout cell lines

Simple one-week method to construct gene-targeting vectors : application to production of human knockout cell lines

Targeted gene disruption is a powerful tool for studying gene function in cells and animals. In addition, this technology includes a potential to correct disease-causing mutations. However, constructing targeting vectors is a laborious step in the gene-targeting strategy, even apart from the low eff...

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Veröffentlicht in:BioTechniques. - 1991. - 41(2006), 3 vom: 15. Sept., Seite 311-6
1. Verfasser: Iiizumi, Susumu (VerfasserIn)
Weitere Verfasser: Nomura, Yuji, So, Sairei, Uegaki, Koichi, Aoki, Kayoko, Shibahara, Kei-ichi, Adachi, Noritaka, Koyama, Hideki
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA Ligases EC 6.5.1.- DNA Ligase ATP EC 6.5.1.1