Simple one-week method to construct gene-targeting vectors : application to production of human knockout cell lines
Targeted gene disruption is a powerful tool for studying gene function in cells and animals. In addition, this technology includes a potential to correct disease-causing mutations. However, constructing targeting vectors is a laborious step in the gene-targeting strategy, even apart from the low eff...
| Publié dans: | BioTechniques. - 1991. - 41(2006), 3 vom: 15. Sept., Seite 311-6 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2006
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA Ligases EC 6.5.1.- DNA Ligase ATP EC 6.5.1.1 |