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|a (NLM)16898140
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Shoji, T
|e verfasserin
|4 aut
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|a Microbial community of biological phosphorus removal process fed with municipal wastewater under different electron acceptor conditions
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|c 2006
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|a Text
|b txt
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|a ohne Hilfsmittel zu benutzen
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|a Date Completed 05.12.2006
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|a Date Revised 17.09.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a The microbial community in a biological phosphorus removal process under different electron acceptor conditions was estimated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) assay and principal-component analysis (PCA). For this purpose, a lab-scale sequencing batch reactor (SBR) fed with municipal wastewater was operated under anaerobic-aerobic, anaerobic-anoxic-aerobic and anaerobic-anoxic conditions. The results of PCR-DGGE targeting the 16S rRNA gene indicated a significant shift in the microbial community with electron acceptor conditions. From the 16S rRNA-based PCA, the microbial shift implies that little oxygen supply caused the deterioration of aerobic bacteria, including aerobic polyphosphate-accumulating organisms (PAOs). Moreover, it also reflects the existence of nitrate-utilizing denitrifiers. On the other hand, although the band patterns of DGGE targeting a functional gene of denitrification (nirS) also showed the microbial shift, the result of PCA differed from that of 16S rRNA-based analysis. There is no conclusive proof that the bacteria represented as the dominant bands detected in the present study are denitrifying-PAOs so far, it should be worthwhile to identify the detected bacteria and to examine their traits as new denitrifying-PAO candidates
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|a Journal Article
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|a DNA Primers
|2 NLM
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|a Industrial Waste
|2 NLM
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|a RNA, Bacterial
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|a RNA, Ribosomal, 16S
|2 NLM
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|a Water Pollutants, Chemical
|2 NLM
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|a Phosphorus
|2 NLM
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|a 27YLU75U4W
|2 NLM
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|a Nittami, T
|e verfasserin
|4 aut
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|a Onuki, M
|e verfasserin
|4 aut
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|a Satoh, H
|e verfasserin
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|a Mino, T
|e verfasserin
|4 aut
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|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 54(2006), 1 vom: 02., Seite 81-9
|w (DE-627)NLM098149431
|x 0273-1223
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|g pages:81-9
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