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DE-627 |
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20231223101635.0 |
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231223s2006 xx ||||| 00| ||eng c |
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|a (NLM)16830985
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Williams, Thomas L
|e verfasserin
|4 aut
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|a Fluorophore-encapsulated solid-supported bilayer vesicles
|b a method for studying membrane permeation processes
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|b nc
|2 rdacarrier
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|a Date Completed 16.08.2007
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|a Date Revised 24.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fatty Acids, Unsaturated
|2 NLM
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|a Fluorescent Dyes
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|a Lipid Bilayers
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|a Phospholipids
|2 NLM
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|a 7,7-dimethyl-5,8-eicosadienoic acid
|2 NLM
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|a 89560-01-0
|2 NLM
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|a Phospholipases A
|2 NLM
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| 650 |
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|a Vareiro, Margarida M L M
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|a Jenkins, A Toby A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 15 vom: 18. Juli, Seite 6473-6
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|x 1520-5827
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|g volume:22
|g year:2006
|g number:15
|g day:18
|g month:07
|g pages:6473-6
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