Fluorophore-encapsulated solid-supported bilayer vesicles : a method for studying membrane permeation processes

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field...

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Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1991. - 22(2006), 15 vom: 18. Juli, Seite 6473-6
Auteur principal: Williams, Thomas L (Auteur)
Autres auteurs: Vareiro, Margarida M L M, Jenkins, A Toby A
Format: Article
Langue:English
Publié: 2006
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, Non-U.S. Gov't Fatty Acids, Unsaturated Fluorescent Dyes Lipid Bilayers Phospholipids 7,7-dimethyl-5,8-eicosadienoic acid 89560-01-0 Phospholipases A EC 3.1.1.32
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Résumé:This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents
Description:Date Completed 16.08.2007
Date Revised 24.11.2016
published: Print
Citation Status MEDLINE
ISSN:0743-7463