Fluorophore-encapsulated solid-supported bilayer vesicles : a method for studying membrane permeation processes

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 15 vom: 18. Juli, Seite 6473-6
1. Verfasser: Williams, Thomas L (VerfasserIn)
Weitere Verfasser: Vareiro, Margarida M L M, Jenkins, A Toby A
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Fatty Acids, Unsaturated Fluorescent Dyes Lipid Bilayers Phospholipids 7,7-dimethyl-5,8-eicosadienoic acid 89560-01-0 Phospholipases A EC 3.1.1.32
Beschreibung
Zusammenfassung:This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents
Beschreibung:Date Completed 16.08.2007
Date Revised 24.11.2016
published: Print
Citation Status MEDLINE
ISSN:1520-5827