Fluorophore-encapsulated solid-supported bilayer vesicles : a method for studying membrane permeation processes
This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 22(2006), 15 vom: 18. Juli, Seite 6473-6 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2006
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Fatty Acids, Unsaturated Fluorescent Dyes Lipid Bilayers Phospholipids 7,7-dimethyl-5,8-eicosadienoic acid 89560-01-0 Phospholipases A EC 3.1.1.32 |
| Résumé: | This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents |
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| Description: | Date Completed 16.08.2007 Date Revised 24.11.2016 published: Print Citation Status MEDLINE |
| ISSN: | 0743-7463 |