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20231223101029.0 |
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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed24n0546.xml
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|a (DE-627)NLM163753180
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|a (NLM)16800689
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Gromelski, Sandra
|e verfasserin
|4 aut
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| 245 |
1 |
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|a DNA condensation and interaction with zwitterionic phospholipids mediated by divalent cations
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| 264 |
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1 |
|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 03.08.2007
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|a Date Revised 24.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Artificial viruses are considered to be a promising tool in gene therapy. To find lipid-DNA complexes with high transfection efficiency but without toxicity is a fundamental aim. Although cationic lipids are frequently toxic for cells, neutral lipids are completely nontoxic. Zwitterionic lipids do not interact with DNA directly; however, the interaction can be mediated by divalent cations. Langmuir monolayers represent a well-defined model system to study the DNA-lipid complexes at the air/water interface (quasi-2D systems). In this work, isotherms, infrared reflection absorption spectroscopy (IRRAS), X-ray reflectivity (XR), grazing incidence X-ray diffraction (GIXD), and Brewster angle microscopy (BAM) measurements are used to study the interaction of calf thymus DNA with DMPE (1,2-dimyristoyl-phosphatidylethanolamine) monolayers mediated by Ca2+ or Mg2+ ions. DNA adsorption is observed only in the presence of divalent cations. At low lateral pressure, the DNA partially penetrates into the lipid monolayer but is squeezed out at high pressure. The adsorption layer has a thickness of 18-19 A. Additionally, GIXD provides information about a one-dimensional ordering of adsorbed DNA. The periodic distance between DNA strands depends on the type of the divalent cation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Cations, Divalent
|2 NLM
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|a Membranes, Artificial
|2 NLM
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7 |
|a Phosphatidylethanolamines
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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| 650 |
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|a calf thymus DNA
|2 NLM
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| 650 |
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|a 91080-16-9
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 650 |
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|a 1,2-dimyristoylphosphatidylethanolamine
|2 NLM
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| 650 |
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7 |
|a Z37OX1ASNK
|2 NLM
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| 700 |
1 |
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|a Brezesinski, Gerald
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 14 vom: 04. Juli, Seite 6293-301
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:22
|g year:2006
|g number:14
|g day:04
|g month:07
|g pages:6293-301
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|d 22
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