Genotype of wild-type measles viruses circulating in Beijing in 2003
OBJECTIVE: To investigate genotype of wild-type measles viruses circulated in Beijing in 2003
| Veröffentlicht in: | Zhonghua er ke za zhi = Chinese journal of pediatrics. - 1960. - 44(2006), 5 vom: 19. Mai, Seite 364-8 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Zhonghua er ke za zhi = Chinese journal of pediatrics |
| Schlagworte: | Journal Article Immunoglobulin M Nucleocapsid Proteins Nucleoproteins RNA, Viral Viral Proteins nucleoprotein, Measles virus |
| Zusammenfassung: | OBJECTIVE: To investigate genotype of wild-type measles viruses circulated in Beijing in 2003 METHODS: Throat swabs specimens were collected from patients seen during an outbreak of measles and from clinically suspected sporadic measles patients in 2003. Vero/SLAM cell lines recommended by WHO were used to isolate measles virus. Four hundreds and fifty nucleotides of COOH-terminal of nucleoprotein (N) genes were amplified by using PR-PCR. The amplified products were sequenced and the sequences were compared with references viruses from GeneBank RESULTS: Eight strains of measles viruses were isolated from throat swabs of patients who came from seven districts and counties of Beijing. Sequence analysis of the 450 nucleotides of COOH-terminal of nucleoprotein (N) genes indicated that these 8 strains belonged to H1a genotype. The average genetic distances of these 8 strains to H1a genotype, Chin9322, H1b genotype, Chin9475 and H1c genotype, Chin9427, were 0.004 - 0.011, 0.026 - 0.031 and 0.015 - 0.022, respectively. The average genetic distances of these 8 strains to H1a genotype, Anhui 01 - 1/Anhui 02 - 2, were 0.000 - 0.009 (0 - 5 nucleotide variation) CONCLUSIONS: Major genotypes of wild-type measles viruses circulated in Beijing in 2003 were H1a genotype. The genotypes H1c, H1b and H2 may have disappeared in Beijing |
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| Beschreibung: | Date Completed 20.07.2010 Date Revised 09.12.2020 published: Print Citation Status MEDLINE |
| ISSN: | 0578-1310 |