The contribution of extensin network formation to rapid, hydrogen peroxide-mediated increases in grapevine callus wall resistance to fungal lytic enzymes

Grapevine (Vitis vinifera cv. Touriga) callus cell walls contain a high level of the monomeric extensin, GvP1. Hydrogen peroxide stimulus of these cultures causes the rapid loss of monomeric GvP1, concomitant with marked increases in insoluble GvP1 amino acids and wall resistance to digestion by fun...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 57(2006), 9 vom: 15., Seite 2025-35
1. Verfasser: Ribeiro, J M (VerfasserIn)
Weitere Verfasser: Pereira, C Silva, Soares, N C, Vieira, A M, Feijó, J A, Jackson, P A
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Glycoproteins Plant Proteins extensin protein, plant Hydrogen Peroxide BBX060AN9V
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245 1 4 |a The contribution of extensin network formation to rapid, hydrogen peroxide-mediated increases in grapevine callus wall resistance to fungal lytic enzymes 
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520 |a Grapevine (Vitis vinifera cv. Touriga) callus cell walls contain a high level of the monomeric extensin, GvP1. Hydrogen peroxide stimulus of these cultures causes the rapid loss of monomeric GvP1, concomitant with marked increases in insoluble GvP1 amino acids and wall resistance to digestion by fungal lytic enzymes. JIM11 immunolocalization studies indicated that monomeric and network GvP1 were evenly distributed in the callus cell wall. These primary cell walls were used to investigate the specific contribution of extensin and other ionically bound cell-wall proteins to hydrogen peroxide-mediated increases in resistance to fungal lytic enzymes. This was performed by removing ionically-bound proteins and assaying for hydrogen peroxide-enhanced resistance after the addition of selected protein fractions. The results indicate that hydrogen peroxide-induced increases in resistance to digestion by fungal lytic enzymes require a co-operative action between network extensin formation and the electrostatic interaction of additional wall proteins with the extracellular matrix 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Plant Proteins  |2 NLM 
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700 1 |a Pereira, C Silva  |e verfasserin  |4 aut 
700 1 |a Soares, N C  |e verfasserin  |4 aut 
700 1 |a Vieira, A M  |e verfasserin  |4 aut 
700 1 |a Feijó, J A  |e verfasserin  |4 aut 
700 1 |a Jackson, P A  |e verfasserin  |4 aut 
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