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20231223091322.0 |
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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed24n0537.xml
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|a (DE-627)NLM161021999
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|a (NLM)16510515
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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100 |
1 |
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|a Murthy, Meena
|e verfasserin
|4 aut
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245 |
1 |
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|a Cation currents in protoplasts from the roots of a Na+ hyperaccumulating mutant of Capsicum annuum
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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500 |
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|a Date Completed 07.06.2006
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A wilty mutant (scabrous diminutive, sd) of Capsicum annuum L. hyperaccumulates Na+ in all tissues and has a lower K+ content in the roots. This has been shown to be due to a greater efflux of (86)Rb+ (K+) and influx of (22)Na+ in the mutant. In this study, the transporters responsible for these fluxes were investigated by applying patch clamp techniques to protoplasts derived from root cortical cells. Inwardly rectifying K+ currents were comparable in the two genotypes, but a characteristically bigger outward K+ current was observed in protoplasts from mutant roots, correlating with a bigger efflux of (86)Rb+ from mutant plants. Whole-cell currents due to the movement of Na+ have also been studied in both genotypes. The magnitude of the time-independent inward currents that conduct Na+ at hyperpolarizing voltages were comparable in both genotypes. However, microelectrode measurements of membrane potentials in cortical cells of roots in high Na+ conditions revealed that the membrane potentials of the root cells in the mutants were approximately 60 mV more negative than in wild-type root cells. Quantitatively, this hyperpolarization is calculated to be sufficient to account for the increased Na+ influx in the mutants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cation Transport Proteins
|2 NLM
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|a Cations
|2 NLM
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|a HKT1 protein, plant
|2 NLM
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|a Ion Channels
|2 NLM
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7 |
|a Plant Proteins
|2 NLM
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7 |
|a Symporters
|2 NLM
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7 |
|a Sodium
|2 NLM
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7 |
|a 9NEZ333N27
|2 NLM
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650 |
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7 |
|a Potassium
|2 NLM
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7 |
|a RWP5GA015D
|2 NLM
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700 |
1 |
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|a Tester, Mark
|e verfasserin
|4 aut
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773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 57(2006), 5 vom: 15., Seite 1171-80
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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773 |
1 |
8 |
|g volume:57
|g year:2006
|g number:5
|g day:15
|g pages:1171-80
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 57
|j 2006
|e 5
|b 15
|h 1171-80
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