Cation currents in protoplasts from the roots of a Na+ hyperaccumulating mutant of Capsicum annuum
A wilty mutant (scabrous diminutive, sd) of Capsicum annuum L. hyperaccumulates Na+ in all tissues and has a lower K+ content in the roots. This has been shown to be due to a greater efflux of (86)Rb+ (K+) and influx of (22)Na+ in the mutant. In this study, the transporters responsible for these flu...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 57(2006), 5 vom: 15., Seite 1171-80 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Cation Transport Proteins Cations HKT1 protein, plant Ion Channels Plant Proteins Symporters Sodium 9NEZ333N27 mehr... |
| Zusammenfassung: | A wilty mutant (scabrous diminutive, sd) of Capsicum annuum L. hyperaccumulates Na+ in all tissues and has a lower K+ content in the roots. This has been shown to be due to a greater efflux of (86)Rb+ (K+) and influx of (22)Na+ in the mutant. In this study, the transporters responsible for these fluxes were investigated by applying patch clamp techniques to protoplasts derived from root cortical cells. Inwardly rectifying K+ currents were comparable in the two genotypes, but a characteristically bigger outward K+ current was observed in protoplasts from mutant roots, correlating with a bigger efflux of (86)Rb+ from mutant plants. Whole-cell currents due to the movement of Na+ have also been studied in both genotypes. The magnitude of the time-independent inward currents that conduct Na+ at hyperpolarizing voltages were comparable in both genotypes. However, microelectrode measurements of membrane potentials in cortical cells of roots in high Na+ conditions revealed that the membrane potentials of the root cells in the mutants were approximately 60 mV more negative than in wild-type root cells. Quantitatively, this hyperpolarization is calculated to be sufficient to account for the increased Na+ influx in the mutants |
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| Beschreibung: | Date Completed 07.06.2006 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1460-2431 |