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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed24n0534.xml
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|a (DE-627)NLM160269806
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|a (NLM)16430295
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Gohon, Yann
|e verfasserin
|4 aut
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| 245 |
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|a Well-defined nanoparticles formed by hydrophobic assembly of a short and polydisperse random terpolymer, amphipol A8-35
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 01.05.2007
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|a Date Revised 24.01.2006
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|a published: Print
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|a Citation Status MEDLINE
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|a Amphipols are short amphilic polymers designed for applications in membrane biochemistry and biophysics and used, in particular, to stabilize membrane proteins in aqueous solutions. Amphipol A8-35 was obtained by modification of a short-chain parent polymer (poly(acrylic acid); PAA) with octyl- and isopropylamine, to yield an amphiphilic product with an average molar mass of 9-10 kg x mol(-1) (sodium salt form) and a polydispersity index of 2.0 to 3.1, depending on the source of PAA. The behavior of A8-35 in aqueous buffers was studied by size exclusion chromatography, static and dynamic light scattering, equilibrium and sedimentation velocity analytical ultracentrifugation, and small angle neutron scattering. Despite the variable length of the chains and the random distribution of hydrophobic groups along them, A8-35 self-organizes into well-defined assemblies. The data are best compatible with most of the polymer forming compact assemblies (particles) with a molar mass of approximately 40 kg x mol(-1), a radius of gyration of approximately 2.4 nm, and a Stokes radius of approximately 3.15 nm. Each particle contains, on average, four A8-35 macromolecules and 75-80 octyl chains. Neutron scattering reveals a sharp interface between the particles and water. A minor (approximately 0.1%) mass fraction of the material forms much larger aggregates, whose proportion may increase under certain conditions of preparation or handling, such as low pH. They can be removed by gel filtration
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Polymers
|2 NLM
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1 |
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|a Giusti, Fabrice
|e verfasserin
|4 aut
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| 700 |
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|a Prata, Carla
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Charvolin, Delphine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Timmins, Peter
|e verfasserin
|4 aut
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| 700 |
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|a Ebel, Christine
|e verfasserin
|4 aut
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| 700 |
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|a Tribet, Christophe
|e verfasserin
|4 aut
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| 700 |
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|a Popot, Jean-Luc
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 3 vom: 31. Jan., Seite 1281-90
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:22
|g year:2006
|g number:3
|g day:31
|g month:01
|g pages:1281-90
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