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20231223083618.0 |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0531.xml
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|a (DE-627)NLM159171261
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|a (NLM)16312959
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kim, Y
|e verfasserin
|4 aut
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| 245 |
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|a Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 12.07.2006
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a Aerobic cometabolism of cis-1,2-dichloroethylene (c-DCE) by a butane-grown mixed culture was evaluated in batch kinetic tests. The transformation of c-DCE resulted in the coincident generation of c-DCE epoxide. Chloride release studies showed approximately 75% oxidative dechlorination of c-DCE. Mass spectrometry confirmed the presence of a compound with mass-to-charge-fragment ratios of 112, 83, 48, and 35. These values are in agreement with the spectra of chemically synthesized c-DCE epoxide. The transformation of c-DCE required O2, was inhibited by butane and was inactivated by acetylene (a known monooxygenase inactivator), indicating that a butane monooxygenase enzyme was likely involved in the transformation of c-DCE. This study showed c-DCE epoxide was biologically transformed, likely by a butane monooxygenase enzyme. c-DCE epoxide transformation was inhibited by both acetylene and c-DCE indicating a monooxygenase enzyme was involved. The epoxide transformation was also stopped when mercuric chloride (HgCl2) was added as a biological inhibitor, further support a biological transformation. To our knowledge this is the first report of the biological transform c-DCE epoxide by a butane-grown culture
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Butanes
|2 NLM
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|a Chlorides
|2 NLM
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|a Dichloroethylenes
|2 NLM
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|a Epoxy Compounds
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|a Water Pollutants, Chemical
|2 NLM
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| 650 |
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|a butane
|2 NLM
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|a 6LV4FOR43R
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|a Mixed Function Oxygenases
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|a EC 1.-
|2 NLM
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|a Acetylene
|2 NLM
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|a OC7TV75O83
|2 NLM
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| 650 |
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|a 1,2-dichloroethylene
|2 NLM
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| 650 |
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|a XU9RUA6YUT
|2 NLM
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| 700 |
1 |
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|a Semprini, L
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 52(2005), 8 vom: 24., Seite 125-31
|w (DE-627)NLM098149431
|x 0273-1223
|7 nnns
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| 773 |
1 |
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|g volume:52
|g year:2005
|g number:8
|g day:24
|g pages:125-31
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 52
|j 2005
|e 8
|b 24
|h 125-31
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