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|a pubmed24n0525.xml
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|a (DE-627)NLM157490483
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|a (NLM)16131507
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Mathieu, Sandrine
|e verfasserin
|4 aut
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|a A carotenoid cleavage dioxygenase from Vitis vinifera L.
|b functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 02.12.2005
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|a Date Revised 10.06.2010
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C(13)-norisoprenoidic compound, and a C(14)-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C(13)-norisoprenoid level increased from véraison to maturity in both cultivars
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbon Isotopes
|2 NLM
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|a DNA, Complementary
|2 NLM
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|a Norisoprenoids
|2 NLM
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|a Dioxygenases
|2 NLM
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|a EC 1.13.11.-
|2 NLM
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|a carotenoid cleavage dioxygenase 1
|2 NLM
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|a EC 1.13.11.-
|2 NLM
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|a Terrier, Nancy
|e verfasserin
|4 aut
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|a Procureur, Jérôme
|e verfasserin
|4 aut
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|a Bigey, Frédéric
|e verfasserin
|4 aut
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|a Günata, Ziya
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 420 vom: 04. Okt., Seite 2721-31
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:56
|g year:2005
|g number:420
|g day:04
|g month:10
|g pages:2721-31
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 56
|j 2005
|e 420
|b 04
|c 10
|h 2721-31
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