A carotenoid cleavage dioxygenase from Vitis vinifera L. : functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation

A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxa...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 56(2005), 420 vom: 04. Okt., Seite 2721-31
1. Verfasser: Mathieu, Sandrine (VerfasserIn)
Weitere Verfasser: Terrier, Nancy, Procureur, Jérôme, Bigey, Frédéric, Günata, Ziya
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Carbon Isotopes DNA, Complementary Norisoprenoids Dioxygenases EC 1.13.11.- carotenoid cleavage dioxygenase 1
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245 1 2 |a A carotenoid cleavage dioxygenase from Vitis vinifera L.  |b functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation 
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520 |a A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C(13)-norisoprenoidic compound, and a C(14)-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C(13)-norisoprenoid level increased from véraison to maturity in both cultivars 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Dioxygenases  |2 NLM 
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700 1 |a Procureur, Jérôme  |e verfasserin  |4 aut 
700 1 |a Bigey, Frédéric  |e verfasserin  |4 aut 
700 1 |a Günata, Ziya  |e verfasserin  |4 aut 
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