A carotenoid cleavage dioxygenase from Vitis vinifera L. : functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation
A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxa...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 56(2005), 420 vom: 04. Okt., Seite 2721-31 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Carbon Isotopes DNA, Complementary Norisoprenoids Dioxygenases EC 1.13.11.- carotenoid cleavage dioxygenase 1 |
| Zusammenfassung: | A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C(13)-norisoprenoidic compound, and a C(14)-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C(13)-norisoprenoid level increased from véraison to maturity in both cultivars |
|---|---|
| Beschreibung: | Date Completed 02.12.2005 Date Revised 10.06.2010 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1460-2431 |