Iron acquisition mechanisms of Flavobacterium psychrophilum

Forty strains of Flavobacterium psychrophilum were tested for the production of siderophores using the universal Chrome Azurol S (CAS) assay. The majority of the strains (85%) were CAS positive (CAS+) and some (15%) were CAS negative (CAS-). The cryptic plasmid pCP1 was carried by all positive strai...

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Veröffentlicht in:Journal of fish diseases. - 1998. - 28(2005), 7 vom: 15. Juli, Seite 391-8
1. Verfasser: Møller, J D (VerfasserIn)
Weitere Verfasser: Ellis, A E, Barnes, A C, Dalsgaard, I
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Comparative Study Journal Article Hydroxybenzoates Siderophores chrome azurol S 1667-99-8 2,2'-Dipyridyl 551W113ZEP Iron E1UOL152H7
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245 1 0 |a Iron acquisition mechanisms of Flavobacterium psychrophilum 
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520 |a Forty strains of Flavobacterium psychrophilum were tested for the production of siderophores using the universal Chrome Azurol S (CAS) assay. The majority of the strains (85%) were CAS positive (CAS+) and some (15%) were CAS negative (CAS-). The cryptic plasmid pCP1 was carried by all positive strains and was lacking from negative strains. While a weak catechol reaction was detectable in CAS+ culture supernatants, the CAS reaction was, to some extent, heat sensitive, questioning whether the positive reaction was caused only by siderophores. The ability to grow in vitro under iron-restricted conditions did not correlate with the CAS reactivity, as growth of both CAS+ and CAS- strains was similarly impaired under iron restriction induced by 2,2 dipyridyl. Suppressed growth under these conditions was restored by addition of FeCl3, haemoglobin and transferrin for both CAS+ and CAS- strains 
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650 4 |a Journal Article 
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700 1 |a Barnes, A C  |e verfasserin  |4 aut 
700 1 |a Dalsgaard, I  |e verfasserin  |4 aut 
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