Iron acquisition mechanisms of Flavobacterium psychrophilum
Forty strains of Flavobacterium psychrophilum were tested for the production of siderophores using the universal Chrome Azurol S (CAS) assay. The majority of the strains (85%) were CAS positive (CAS+) and some (15%) were CAS negative (CAS-). The cryptic plasmid pCP1 was carried by all positive strai...
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 28(2005), 7 vom: 15. Juli, Seite 391-8 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Comparative Study Journal Article Hydroxybenzoates Siderophores chrome azurol S 1667-99-8 2,2'-Dipyridyl 551W113ZEP Iron E1UOL152H7 |
| Zusammenfassung: | Forty strains of Flavobacterium psychrophilum were tested for the production of siderophores using the universal Chrome Azurol S (CAS) assay. The majority of the strains (85%) were CAS positive (CAS+) and some (15%) were CAS negative (CAS-). The cryptic plasmid pCP1 was carried by all positive strains and was lacking from negative strains. While a weak catechol reaction was detectable in CAS+ culture supernatants, the CAS reaction was, to some extent, heat sensitive, questioning whether the positive reaction was caused only by siderophores. The ability to grow in vitro under iron-restricted conditions did not correlate with the CAS reactivity, as growth of both CAS+ and CAS- strains was similarly impaired under iron restriction induced by 2,2 dipyridyl. Suppressed growth under these conditions was restored by addition of FeCl3, haemoglobin and transferrin for both CAS+ and CAS- strains |
|---|---|
| Beschreibung: | Date Completed 21.11.2005 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1365-2761 |