QCM-D on mica for parallel QCM-D-AFM studies

Quartz crystal microbalance with dissipation monitoring (QCM-D) has developed into a recognized method to study adsorption processes in liquid, such as the formation of supported lipid bilayers and protein adsorption. However, the large intrinsic roughness of currently used gold-coated or silica-coa...

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Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 20(2004), 11 vom: 25. Mai, Seite 4609-13
Auteur principal: Richter, Ralf P (Auteur)
Autres auteurs: Brisson, Alain
Format: Article
Langue:English
Publié: 2004
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Aluminum Silicates Annexin A5 Lipid Bilayers Phosphatidylcholines Phosphatidylserines Water 059QF0KO0R Quartz 14808-60-7 plus... 1,2-dioleoylphosphatidylserine 70614-14-1 1,2-oleoylphosphatidylcholine EDS2L3ODLV mica V8A1AW0880
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245 1 0 |a QCM-D on mica for parallel QCM-D-AFM studies 
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520 |a Quartz crystal microbalance with dissipation monitoring (QCM-D) has developed into a recognized method to study adsorption processes in liquid, such as the formation of supported lipid bilayers and protein adsorption. However, the large intrinsic roughness of currently used gold-coated or silica-coated QCM-D sensors limits parallel structural characterization by atomic force microscopy (AFM). We present a method for coating QCM-D sensors with thin mica sheets operating in liquid with high stability and sensitivity. We define criteria to objectively assess the reliability of the QCM-D measurements and demonstrate that the mica-coated sensors can be used to follow the formation of supported lipid membranes and subsequent protein adsorption. This method allows combining QCM-D and AFM investigations on identical supports, providing detailed physicochemical and structural characterization of model membranes 
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650 7 |a Phosphatidylserines  |2 NLM 
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650 7 |a mica  |2 NLM 
650 7 |a V8A1AW0880  |2 NLM 
700 1 |a Brisson, Alain  |e verfasserin  |4 aut 
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