QCM-D on mica for parallel QCM-D-AFM studies
Quartz crystal microbalance with dissipation monitoring (QCM-D) has developed into a recognized method to study adsorption processes in liquid, such as the formation of supported lipid bilayers and protein adsorption. However, the large intrinsic roughness of currently used gold-coated or silica-coa...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 20(2004), 11 vom: 25. Mai, Seite 4609-13 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Aluminum Silicates Annexin A5 Lipid Bilayers Phosphatidylcholines Phosphatidylserines Water 059QF0KO0R Quartz 14808-60-7 mehr... |
| Zusammenfassung: | Quartz crystal microbalance with dissipation monitoring (QCM-D) has developed into a recognized method to study adsorption processes in liquid, such as the formation of supported lipid bilayers and protein adsorption. However, the large intrinsic roughness of currently used gold-coated or silica-coated QCM-D sensors limits parallel structural characterization by atomic force microscopy (AFM). We present a method for coating QCM-D sensors with thin mica sheets operating in liquid with high stability and sensitivity. We define criteria to objectively assess the reliability of the QCM-D measurements and demonstrate that the mica-coated sensors can be used to follow the formation of supported lipid membranes and subsequent protein adsorption. This method allows combining QCM-D and AFM investigations on identical supports, providing detailed physicochemical and structural characterization of model membranes |
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| Beschreibung: | Date Completed 12.01.2006 Date Revised 26.10.2019 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |