The effect of growth regulators and sucrose on anthocyanin production in Camptotheca acuminata cell cultures

The effect of different concentrations of growth regulators and sucrose on anthocyanin production in cell suspension cultures of Camptotheca acuminata Decaisne (Nyssaceae) was described for the first time and qualitatively and quantitatively evaluated. Anthocyanin production was significantly greate...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 43(2005), 3 vom: 15. März, Seite 293-8
1. Verfasser: Pasqua, Gabriella (VerfasserIn)
Weitere Verfasser: Monacelli, Barbara, Mulinacci, Nadia, Rinaldi, Simona, Giaccherini, Catia, Innocenti, Marzia, Vinceri, Franco Francesco
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Comparative Study Journal Article Anthocyanins Plant Growth Regulators Sweetening Agents Sucrose 57-50-1
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520 |a The effect of different concentrations of growth regulators and sucrose on anthocyanin production in cell suspension cultures of Camptotheca acuminata Decaisne (Nyssaceae) was described for the first time and qualitatively and quantitatively evaluated. Anthocyanin production was significantly greater in the presence of kinetin, compared to benzyladenine, with the greatest concentration observed in the presence of 2 microM kinetin. No significant differences in anthocyanin production were observed when comparing 2,4-dichlorophenoxyacetic acid to alpha-naphthaleneacetic acid, except when using 2 microM, 2,4-dichlorophenoxyacetic acid, which resulted in greater anthocyanin production. High sucrose concentration enhanced the production of anthocyanins. Based on the absence of anthocyanin production in the dark, we concluded that light was essential for stimulating anthocyanin production. The optimised medium consisted of: 2 microM kinetin, 2 microM 2,4-dichlorophenoxyacetic acid and 292 mM sucrose. HPLC/DAD and HPLC/MS analyses revealed that the main anthocyanin was Cy 3-O-galactoside and that the minor derivative was Cy 3-O-glucoside 
650 4 |a Comparative Study 
650 4 |a Journal Article 
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700 1 |a Monacelli, Barbara  |e verfasserin  |4 aut 
700 1 |a Mulinacci, Nadia  |e verfasserin  |4 aut 
700 1 |a Rinaldi, Simona  |e verfasserin  |4 aut 
700 1 |a Giaccherini, Catia  |e verfasserin  |4 aut 
700 1 |a Innocenti, Marzia  |e verfasserin  |4 aut 
700 1 |a Vinceri, Franco Francesco  |e verfasserin  |4 aut 
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