Regiospecific control of protein expression in cells cultured on two-component counter gradients of extracellular matrix proteins
This work describes the use of microfluidic tools to generate covalently immobilized counter gradients of extracellular matrix (ECM) proteins laminin and collagen I. Using these platforms, we demonstrate control of the expression levels of two proteins linked to cell cycle progression by virtue of t...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 21(2005), 7 vom: 29. März, Seite 3061-8 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Biomarkers Collagen Type I Laminin Proliferating Cell Nuclear Antigen |
| Zusammenfassung: | This work describes the use of microfluidic tools to generate covalently immobilized counter gradients of extracellular matrix (ECM) proteins laminin and collagen I. Using these platforms, we demonstrate control of the expression levels of two proteins linked to cell cycle progression by virtue of the spatial location of cells on the gradients, and hence by the local ECM environments in these devices. In contrast to physisorbed gradients, covalently immobilized protein patterns preserved the gradient fidelity, making long term cell studies feasible. This method of precisely controlling local cell environments is simple and broadly portable to other cell types and to other ECM proteins or soluble factors. Our approach promises to enable new investigations in cell biology that will contribute to the establishment of biological design rules for controlling cell growth, differentiation, and function |
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| Beschreibung: | Date Completed 14.06.2006 Date Revised 10.03.2022 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |