Localization of ascorbic acid, ascorbic acid oxidase, and glutathione in roots of Cucurbita maxima L

To understand the function of ascorbic acid (ASC) in root development, the distribution of ASC, ASC oxidase, and glutathione (GSH) were investigated in cells and tissues of the root apex of Cucubita maxima. ASC was regularly distributed in the cytosol of almost all root cells, with the exception of...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 55(2004), 408 vom: 14. Dez., Seite 2589-97
1. Verfasser: Liso, Rosalia (VerfasserIn)
Weitere Verfasser: De Tullio, Mario C, Ciraci, Samantha, Balestrini, Raffaella, La Rocca, Nicoletta, Bruno, Leonardo, Chiappetta, Adriana, Bitonti, Maria Beatrice, Bonfante, Paola, Arrigoni, Oreste
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Amaryllidaceae Alkaloids Phenanthridines Sugar Acids galactonolactone 2426-46-2 Ascorbate Oxidase EC 1.10.3.3 Glutathione GAN16C9B8O mehr... lycorine I9Q105R5BU Ascorbic Acid PQ6CK8PD0R Dehydroascorbic Acid Y2Z3ZTP9UM
LEADER 01000naa a22002652 4500
001 NLM151893225
003 DE-627
005 20231223060914.0
007 tu
008 231223s2004 xx ||||| 00| ||eng c
028 5 2 |a pubmed24n0506.xml 
035 |a (DE-627)NLM151893225 
035 |a (NLM)15520029 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Liso, Rosalia  |e verfasserin  |4 aut 
245 1 0 |a Localization of ascorbic acid, ascorbic acid oxidase, and glutathione in roots of Cucurbita maxima L 
264 1 |c 2004 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 03.02.2005 
500 |a Date Revised 19.11.2015 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a To understand the function of ascorbic acid (ASC) in root development, the distribution of ASC, ASC oxidase, and glutathione (GSH) were investigated in cells and tissues of the root apex of Cucubita maxima. ASC was regularly distributed in the cytosol of almost all root cells, with the exception of quiescent centre (QC) cells. ASC also occurred at the surface of the nuclear membrane and correspondingly in the nucleoli. No ASC could be observed in vacuoles. ASC oxidase was detected by immunolocalization mainly in cell walls and vacuoles. This enzyme was particularly abundant in the QC and in differentiating vascular tissues and was absent in lateral root primordia. Administration of the ASC precursor L-galactono-gamma-lactone markedly increased ASC content in all root cells, including the QC. Root treatment with the ASC oxidized product, dehydroascorbic acid (DHA), also increased ASC content, but caused ASC accumulation only in peripheral tissues, where DHA was apparently reduced at the expense of GSH. The different pattern of distribution of ASC in different tissues and cell compartments reflects its possible role in cell metabolism and root morphogenesis 
650 4 |a Journal Article 
650 7 |a Amaryllidaceae Alkaloids  |2 NLM 
650 7 |a Phenanthridines  |2 NLM 
650 7 |a Sugar Acids  |2 NLM 
650 7 |a galactonolactone  |2 NLM 
650 7 |a 2426-46-2  |2 NLM 
650 7 |a Ascorbate Oxidase  |2 NLM 
650 7 |a EC 1.10.3.3  |2 NLM 
650 7 |a Glutathione  |2 NLM 
650 7 |a GAN16C9B8O  |2 NLM 
650 7 |a lycorine  |2 NLM 
650 7 |a I9Q105R5BU  |2 NLM 
650 7 |a Ascorbic Acid  |2 NLM 
650 7 |a PQ6CK8PD0R  |2 NLM 
650 7 |a Dehydroascorbic Acid  |2 NLM 
650 7 |a Y2Z3ZTP9UM  |2 NLM 
700 1 |a De Tullio, Mario C  |e verfasserin  |4 aut 
700 1 |a Ciraci, Samantha  |e verfasserin  |4 aut 
700 1 |a Balestrini, Raffaella  |e verfasserin  |4 aut 
700 1 |a La Rocca, Nicoletta  |e verfasserin  |4 aut 
700 1 |a Bruno, Leonardo  |e verfasserin  |4 aut 
700 1 |a Chiappetta, Adriana  |e verfasserin  |4 aut 
700 1 |a Bitonti, Maria Beatrice  |e verfasserin  |4 aut 
700 1 |a Bonfante, Paola  |e verfasserin  |4 aut 
700 1 |a Arrigoni, Oreste  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g 55(2004), 408 vom: 14. Dez., Seite 2589-97  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnns 
773 1 8 |g volume:55  |g year:2004  |g number:408  |g day:14  |g month:12  |g pages:2589-97 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 55  |j 2004  |e 408  |b 14  |c 12  |h 2589-97