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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed25n0504.xml
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|a (DE-627)NLM151219273
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|a (NLM)15448175
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Mojović, Milos
|e verfasserin
|4 aut
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|a Oxygen radicals produced by plant plasma membranes
|b an EPR spin-trap study
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|b nc
|2 rdacarrier
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|a Date Completed 03.02.2005
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|a Date Revised 15.11.2006
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spin-trap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cyclic N-Oxides
|2 NLM
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|a Superoxides
|2 NLM
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|a 11062-77-4
|2 NLM
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|a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide
|2 NLM
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|a 157230-67-6
|2 NLM
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|a Hydroxyl Radical
|2 NLM
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|a 3352-57-6
|2 NLM
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|a Vuletić, Mirjana
|e verfasserin
|4 aut
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|a Bacić, Goran G
|e verfasserin
|4 aut
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|a Vucinić, Zeljko
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 408 vom: 10. Dez., Seite 2523-31
|w (DE-627)NLM098182706
|x 0022-0957
|7 nnns
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| 773 |
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|g volume:55
|g year:2004
|g number:408
|g day:10
|g month:12
|g pages:2523-31
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|d 55
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