Oxygen radicals produced by plant plasma membranes : an EPR spin-trap study
Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute....
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 55(2004), 408 vom: 10. Dez., Seite 2523-31 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Cyclic N-Oxides Superoxides 11062-77-4 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide 157230-67-6 Hydroxyl Radical 3352-57-6 |
| Zusammenfassung: | Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spin-trap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition |
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| Beschreibung: | Date Completed 03.02.2005 Date Revised 15.11.2006 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0022-0957 |