Overexpression of indoleamine 2,3-dioxygenase in human inflammatory bowel disease

T-cells are causally involved in the pathogenesis of inflammatory bowel disease (IBD). The tryptophan-metabolizing enzyme indoleamine 2,3-dioxygenase (IDO) regulates T-cell proliferation and survival. We show in this report that IDO mRNA is markedly induced in lesional colonic biopsies of IBD patien...

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Bibliographische Detailangaben
Veröffentlicht in:	Clinical immunology (Orlando, Fla.). - 1999. - 113(2004), 1 vom: 15. Okt., Seite 47-55
1. Verfasser:	Wolf, Anna Maria (VerfasserIn)
Weitere Verfasser:	Wolf, Dominik, Rumpold, Holger, Moschen, Alexander R, Kaser, Arthur, Obrist, Peter, Fuchs, Dietmar, Brandacher, Gerald, Winkler, Christiane, Geboes, Karel, Rutgeerts, Paul, Tilg, Herbert
Format:	Aufsatz
Sprache:	English
Veröffentlicht:	2004
Zugriff auf das übergeordnete Werk:	Clinical immunology (Orlando, Fla.)
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Antibodies, Monoclonal Gastrointestinal Agents Histocompatibility Antigens Class II IL3RA protein, human Indoleamine-Pyrrole 2,3,-Dioxygenase Interleukin-3 Receptor alpha Subunit RNA, Messenger Receptors, Interleukin-3 mehr... Tumor Necrosis Factor-alpha Infliximab B72HH48FLU Tryptophan Oxygenase EC 1.13.11.11

Beschreibung
Zusammenfassung:	T-cells are causally involved in the pathogenesis of inflammatory bowel disease (IBD). The tryptophan-metabolizing enzyme indoleamine 2,3-dioxygenase (IDO) regulates T-cell proliferation and survival. We show in this report that IDO mRNA is markedly induced in lesional colonic biopsies of IBD patients. IDO is primarily expressed in CD123(+) mononuclear cells infiltrating the submucosal areas of the inflamed lesions. In Crohn's disease (CD), IDO is also strongly expressed in perifollicular regions of lymphoid follicles. Upregulation of IDO is of functional significance, as we detected an increase of kynurenine and of the kynurenine/tryptophan ratio in supernatants from colonic explant cultures (CECs) of CD patients. Immunohistochemistry of colonic biopsies taken from CD patients prior and after treatment with the TNF-blocking antibody Infliximab revealed reduced IDO expression in patients with good clinical response to Infliximab. In summary, high local expression of IDO may represent an anti-inflammatory mechanism tempting to counterbalance the tissue-damaging effects of activated T-cells infiltrating the colonic mucosa in IBD
Beschreibung:	Date Completed 26.10.2004 Date Revised 19.11.2015 published: Print Citation Status MEDLINE
ISSN:	1521-7035