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01000naa a22002652 4500 |
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DE-627 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0500.xml
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|a (DE-627)NLM149902824
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|a (NLM)15310064
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Yoshioka, Hiroyuki
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat
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| 264 |
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1 |
|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 04.11.2004
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Seeds of common buckwheat (Fagopyrum esculentum) contain valuable nutritive substances but also allergenic proteins that cause hypersensitive reactions. Thus, the development of hypoallergenic buckwheat would make this important pseudo-cereal available to allergic people. A major allergenic protein of buckwheat is Fag e 1. We isolated the respective cDNA, coding for a 22 kDa protein, from a recently developed autogamous strain of common buckwheat and confirmed its immunoglobulin E (IgE)-binding activity using recombinant Fag e 1 and sera of allergic patients. The derived amino acid sequence from Fag e 1 cDNA was used to synthesize an overlapping peptide library on nitrocellulose membranes for the determination of the Fag e 1 epitopes. We identified eight epitopes and the critical amino acids for IgE-binding within the epitopes. This epitope analysis of a major allergenic protein of buckwheat should help therapeutic efforts and aid in the development of hypoallergenic buckwheat
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4 |
|a Journal Article
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7 |
|a Allergens
|2 NLM
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| 650 |
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7 |
|a Antigens, Plant
|2 NLM
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| 650 |
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7 |
|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a Epitopes
|2 NLM
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| 650 |
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7 |
|a Fag e 1 allergen, Fagopyrum esculentum
|2 NLM
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| 650 |
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7 |
|a Lymphokines
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Recombinant Proteins
|2 NLM
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| 650 |
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7 |
|a immunoglobulin-binding factors
|2 NLM
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| 700 |
1 |
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|a Ohmoto, Tsuyoshi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Urisu, Atsuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mine, Yoshinori
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Adachi, Taiji
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 161(2004), 7 vom: 17. Juli, Seite 761-7
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
8 |
|g volume:161
|g year:2004
|g number:7
|g day:17
|g month:07
|g pages:761-7
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 161
|j 2004
|e 7
|b 17
|c 07
|h 761-7
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