Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat
Seeds of common buckwheat (Fagopyrum esculentum) contain valuable nutritive substances but also allergenic proteins that cause hypersensitive reactions. Thus, the development of hypoallergenic buckwheat would make this important pseudo-cereal available to allergic people. A major allergenic protein...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 161(2004), 7 vom: 17. Juli, Seite 761-7 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Allergens Antigens, Plant DNA, Complementary Epitopes Fag e 1 allergen, Fagopyrum esculentum Lymphokines Plant Proteins Recombinant Proteins immunoglobulin-binding factors |
| Zusammenfassung: | Seeds of common buckwheat (Fagopyrum esculentum) contain valuable nutritive substances but also allergenic proteins that cause hypersensitive reactions. Thus, the development of hypoallergenic buckwheat would make this important pseudo-cereal available to allergic people. A major allergenic protein of buckwheat is Fag e 1. We isolated the respective cDNA, coding for a 22 kDa protein, from a recently developed autogamous strain of common buckwheat and confirmed its immunoglobulin E (IgE)-binding activity using recombinant Fag e 1 and sera of allergic patients. The derived amino acid sequence from Fag e 1 cDNA was used to synthesize an overlapping peptide library on nitrocellulose membranes for the determination of the Fag e 1 epitopes. We identified eight epitopes and the critical amino acids for IgE-binding within the epitopes. This epitope analysis of a major allergenic protein of buckwheat should help therapeutic efforts and aid in the development of hypoallergenic buckwheat |
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| Beschreibung: | Date Completed 04.11.2004 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1618-1328 |