Rapid routine structure determination of macromolecular assemblies using electron microscopy current progress and further challenges

Rapid routine structure determination of macromolecular assemblies using electron microscopy : current progress and further challenges

Although the methodology of molecular microscopy has enormous potential, it is time consuming and labor intensive. The techniques required to produce a three-dimensional (3D) electron density map of a macromolecular structure normally require manual operation of an electron microscope by a skilled o...

Description complète

Détails bibliographiques
Publié dans:Journal of synchrotron radiation. - 1998. - 11(2004), Pt 1 vom: 01. Jan., Seite 83-5
Auteur principal: Carragher, Bridget (Auteur)
Autres auteurs: Fellmann, Denis, Guerra, Francisco, Milligan, Ronald A, Mouche, Fabrice, Pulokas, James, Sheehan, Brian, Quispe, Joel, Suloway, Christian, Zhu, Yuanxin, Potter, Clinton S
Format: Article
Langue:English
Publié: 2004
Accès à la collection:Journal of synchrotron radiation
Sujets:Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Biopolymers Macromolecular Substances Hemocyanins 9013-72-3
LEADER 01000caa a22002652 4500
001 NLM143615793
003 DE-627
005 20250205045755.0
007 tu
008 231223s2004 xx ||||| 00| ||eng c
028 5 2 |a pubmed25n0479.xml 
035 |a (DE-627)NLM143615793 
035 |a (NLM)14646141 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Carragher, Bridget  |e verfasserin  |4 aut 
245 1 0 |a Rapid routine structure determination of macromolecular assemblies using electron microscopy  |b current progress and further challenges 
264 1 |c 2004 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 15.04.2004 
500 |a Date Revised 10.12.2019 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Although the methodology of molecular microscopy has enormous potential, it is time consuming and labor intensive. The techniques required to produce a three-dimensional (3D) electron density map of a macromolecular structure normally require manual operation of an electron microscope by a skilled operator and manual supervision of the sometimes complex software needed for analysis and calculation of 3D maps. Systems to automate the process of data acquisition from an electron microscope are being developing and these systems are being integrated with specimen handling operations and post acquisition data processing. Here, the current performance of our existing systems and the future challenges involved in substantially improving both the sustained throughput and the yield of automated data collection and analysis are reported 
650 4 |a Evaluation Study 
650 4 |a Journal Article 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Research Support, U.S. Gov't, P.H.S. 
650 7 |a Biopolymers  |2 NLM 
650 7 |a Macromolecular Substances  |2 NLM 
650 7 |a Hemocyanins  |2 NLM 
650 7 |a 9013-72-3  |2 NLM 
700 1 |a Fellmann, Denis  |e verfasserin  |4 aut 
700 1 |a Guerra, Francisco  |e verfasserin  |4 aut 
700 1 |a Milligan, Ronald A  |e verfasserin  |4 aut 
700 1 |a Mouche, Fabrice  |e verfasserin  |4 aut 
700 1 |a Pulokas, James  |e verfasserin  |4 aut 
700 1 |a Sheehan, Brian  |e verfasserin  |4 aut 
700 1 |a Quispe, Joel  |e verfasserin  |4 aut 
700 1 |a Suloway, Christian  |e verfasserin  |4 aut 
700 1 |a Zhu, Yuanxin  |e verfasserin  |4 aut 
700 1 |a Potter, Clinton S  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of synchrotron radiation  |d 1998  |g 11(2004), Pt 1 vom: 01. Jan., Seite 83-5  |w (DE-627)NLM09824129X  |x 0909-0495  |7 nnns 
773 1 8 |g volume:11  |g year:2004  |g number:Pt 1  |g day:01  |g month:01  |g pages:83-5 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_40 
912 |a GBV_ILN_350 
912 |a GBV_ILN_2005 
951 |a AR 
952 |d 11  |j 2004  |e Pt 1  |b 01  |c 01  |h 83-5