Rapid routine structure determination of macromolecular assemblies using electron microscopy : current progress and further challenges
Although the methodology of molecular microscopy has enormous potential, it is time consuming and labor intensive. The techniques required to produce a three-dimensional (3D) electron density map of a macromolecular structure normally require manual operation of an electron microscope by a skilled o...
| Publié dans: | Journal of synchrotron radiation. - 1998. - 11(2004), Pt 1 vom: 01. Jan., Seite 83-5 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2004
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| Accès à la collection: | Journal of synchrotron radiation |
| Sujets: | Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Biopolymers Macromolecular Substances Hemocyanins 9013-72-3 |
| Résumé: | Although the methodology of molecular microscopy has enormous potential, it is time consuming and labor intensive. The techniques required to produce a three-dimensional (3D) electron density map of a macromolecular structure normally require manual operation of an electron microscope by a skilled operator and manual supervision of the sometimes complex software needed for analysis and calculation of 3D maps. Systems to automate the process of data acquisition from an electron microscope are being developing and these systems are being integrated with specimen handling operations and post acquisition data processing. Here, the current performance of our existing systems and the future challenges involved in substantially improving both the sustained throughput and the yield of automated data collection and analysis are reported |
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| Description: | Date Completed 15.04.2004 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0909-0495 |