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231222s2003 xx ||||| 00| ||eng c |
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|a (NLM)12964872
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Iqbal, M Cassim Mohamed
|e verfasserin
|4 aut
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| 245 |
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|a Uptake and distribution of sinigrin in microspore derived embryos of Brassica napus L
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| 264 |
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|c 2003
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 04.12.2003
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a In Brassica napus, glucosinolates are transported from all parts of the plant into the embryo during seed development. In this study we describe the uptake of the alkenyl glucosinolate sinigrin by microspore derived embryos from high and low glucosinolate genotypes. Microspore derived embryos develop completely isolated from maternal tissues unlike zygotic embryos, which contains glucosinolates transported into the embryo synthesised in the vegetative tissues. The sinigrin in the culture medium was almost completely absorbed by the embryos after three days of culture. The embryos of high and low glucosinolate genotypes were equally capable of absorbing sinigrin from the medium. A significant increase in different alkenyl glucosinolates following feeding of sinigrin suggests induction of biosynthetic enzymes in the embryos. Following excess feeding of sinigrin, we found a strong uptake against a concentration gradient and stable accumulation by the embryos. The glucosinolate was detected in single dissected cotyledons by a photometric test and by HPLC. This test could potentially be useful for screening mutants defective in glucosinolate uptake into the embryo
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Glucosinolates
|2 NLM
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| 650 |
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|a sinigrin
|2 NLM
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| 650 |
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|a 50UM64RMBJ
|2 NLM
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| 700 |
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|a Möllers, Christian
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 160(2003), 8 vom: 01. Aug., Seite 961-6
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:160
|g year:2003
|g number:8
|g day:01
|g month:08
|g pages:961-6
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| 952 |
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|d 160
|j 2003
|e 8
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|h 961-6
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