|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM126744734 |
| 003 |
DE-627 |
| 005 |
20231222212524.0 |
| 007 |
tu |
| 008 |
231222s2003 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0423.xml
|
| 035 |
|
|
|a (DE-627)NLM126744734
|
| 035 |
|
|
|a (NLM)12921758
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Lucas, Alexander H
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Molecular ontogeny of the human antibody repertoire to the Haemophilus influenzae type B polysaccharide
|b expression of canonical variable regions and their variants in vaccinated infants
|
| 264 |
|
1 |
|c 2003
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 01.10.2003
|
| 500 |
|
|
|a Date Revised 07.11.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a A structurally conserved antibody combining site, encoded by the IGH V3-23 and kappa A2 variable (V) region gene segments, predominates the adult immune response to the Haemophilus influenzae type b (Hib) capsular polysaccharide (PS). This site has been elevated to canonical status based upon its relative molecular uniformity and prevalence in adults. To date, no studies have examined the primary structure of Hib PS-specific antibodies in young infants, who are the primary targets of Hib vaccination. In this study we show that canonical Hib PS-specific heavy (H) and light (L) chain V regions are present in 4-month-old infants following two vaccinations with Hib PS-protein conjugates. The infant V regions contain sequence polymorphisms that resemble those found in adult antibodies, as well as polymorphisms at position 95a of the A2 L chain not previously observed in adults. In vitro studies of Fab fragments and recombinant IgG2 antibodies using these V regions identify sequence polymorphisms that impact Hib PS binding affinity and bactericidal activity. These results demonstrate the establishment of canonical V regions in early ontogeny and provide a structural explanation of how canonical antibodies in the infant can vary in their affinity and protective activity against Hib
|
| 650 |
|
4 |
|a Comparative Study
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, P.H.S.
|
| 650 |
|
7 |
|a Antibodies, Anti-Idiotypic
|2 NLM
|
| 650 |
|
7 |
|a Antibodies, Bacterial
|2 NLM
|
| 650 |
|
7 |
|a Bacterial Proteins
|2 NLM
|
| 650 |
|
7 |
|a Haemophilus Vaccines
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin Fab Fragments
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin Heavy Chains
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin Light Chains
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin Variable Region
|2 NLM
|
| 650 |
|
7 |
|a Polysaccharides, Bacterial
|2 NLM
|
| 650 |
|
7 |
|a Vaccines, Synthetic
|2 NLM
|
| 650 |
|
7 |
|a HibTITER protein, Haemophilus influenzae
|2 NLM
|
| 650 |
|
7 |
|a 126161-67-9
|2 NLM
|
| 700 |
1 |
|
|a McLean, Gary R
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Reason, Donald C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a O'Connor, Adam P
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Felton, Mistique C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Moulton, Karen D
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 108(2003), 2 vom: 15. Aug., Seite 119-27
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
|
| 773 |
1 |
8 |
|g volume:108
|g year:2003
|g number:2
|g day:15
|g month:08
|g pages:119-27
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_11
|
| 912 |
|
|
|a GBV_ILN_24
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 108
|j 2003
|e 2
|b 15
|c 08
|h 119-27
|