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01000naa a22002652 4500 |
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DE-627 |
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20231222201141.0 |
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231222s2003 xx ||||| 00| ||eng c |
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|a pubmed24n0411.xml
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|a (NLM)12554705
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zanetti, María Eugenia
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Phosphorylation of a member of the MBF1 transcriptional co-activator family, StMBF1, is stimulated in potato cell suspensions upon fungal elicitor challenge
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|c 2003
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 16.06.2003
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|a Date Revised 10.03.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. Previously, it has been described as being up-regulated at the transcriptional level by fungal and abiotic stress. To understand whether StMBF1 is also regulated at the post-translational level, in vitro as well as in vivo phosphorylation assays were performed. StMBF1 is phosphorylated under both experimental conditions and [(32)P] incorporation into StMBF1 increases after treatment of potato cells with hyphal cell wall components (HWC) derived from Phytophthora infestans. The StMBF1-phosphorylating activity is strongly inhibited by the calcium-chelator EGTA and partially inhibited by calmodulin antagonists. Using bacterial purified StMBF1 as a substrate, a 57 kDa calcium-dependent protein kinase (p57) that is able to phosphorylate StMBF1 was detected. The StMBF1 kinase activity of p57 was higher in elicited than in non-treated cells. The role of the elicitor-dependent phosphorylation of StMBF1 is discussed
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Phosphorus Radioisotopes
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Trans-Activators
|2 NLM
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| 650 |
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|a Egtazic Acid
|2 NLM
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| 650 |
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|a 526U7A2651
|2 NLM
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| 650 |
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|a Protein Kinases
|2 NLM
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| 650 |
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|a EC 2.7.-
|2 NLM
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| 650 |
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|a Dimethyl Sulfoxide
|2 NLM
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| 650 |
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|a YOW8V9698H
|2 NLM
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| 700 |
1 |
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|a Blanco, Flavio Antonio
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Daleo, Gustavo Raúl
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Casalongué, Claudia Anahí
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 54(2003), 383 vom: 01. Feb., Seite 623-32
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:54
|g year:2003
|g number:383
|g day:01
|g month:02
|g pages:623-32
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|d 54
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|e 383
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|h 623-32
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