Phosphorylation of a member of the MBF1 transcriptional co-activator family, StMBF1, is stimulated in potato cell suspensions upon fungal elicitor challenge
StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. Previously, it has been described as being up-regulated at the transcriptional level by fungal and abiotic stress. To understand whether StMBF1 is also regulated at the po...
Veröffentlicht in: | Journal of experimental botany. - 1985. - 54(2003), 383 vom: 01. Feb., Seite 623-32 |
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1. Verfasser: | |
Weitere Verfasser: | , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
2003
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Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Phosphorus Radioisotopes Plant Proteins Trans-Activators Egtazic Acid 526U7A2651 Protein Kinases EC 2.7.- Dimethyl Sulfoxide |
Zusammenfassung: | StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. Previously, it has been described as being up-regulated at the transcriptional level by fungal and abiotic stress. To understand whether StMBF1 is also regulated at the post-translational level, in vitro as well as in vivo phosphorylation assays were performed. StMBF1 is phosphorylated under both experimental conditions and [(32)P] incorporation into StMBF1 increases after treatment of potato cells with hyphal cell wall components (HWC) derived from Phytophthora infestans. The StMBF1-phosphorylating activity is strongly inhibited by the calcium-chelator EGTA and partially inhibited by calmodulin antagonists. Using bacterial purified StMBF1 as a substrate, a 57 kDa calcium-dependent protein kinase (p57) that is able to phosphorylate StMBF1 was detected. The StMBF1 kinase activity of p57 was higher in elicited than in non-treated cells. The role of the elicitor-dependent phosphorylation of StMBF1 is discussed |
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Beschreibung: | Date Completed 16.06.2003 Date Revised 10.03.2022 published: Print Citation Status MEDLINE |
ISSN: | 1460-2431 |