Determination of gene copy number and genotype of transgenic Arabidopsis thaliana by competitive PCR
A simple and rapid method is described for determining the integrated T-DNA copy number and the genotype in transgenic Arabidopsis thaliana by two-step competitive PCR. First, the amount of genomic DNA in the extracts, obtained from an individual A. thaliana transformant, was accurately determined b...
| Publié dans: | Journal of experimental botany. - 1985. - 53(2002), 373 vom: 02. Juni, Seite 1515-20 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2002
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| Accès à la collection: | Journal of experimental botany |
| Sujets: | Comparative Study Journal Article DNA, Bacterial DNA, Plant T-DNA 4-Hydroxyphenylpyruvate Dioxygenase EC 1.13.11.27 Citrate (si)-Synthase EC 2.3.3.1 |