Determination of gene copy number and genotype of transgenic Arabidopsis thaliana by competitive PCR

A simple and rapid method is described for determining the integrated T-DNA copy number and the genotype in transgenic Arabidopsis thaliana by two-step competitive PCR. First, the amount of genomic DNA in the extracts, obtained from an individual A. thaliana transformant, was accurately determined b...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 53(2002), 373 vom: 02. Juni, Seite 1515-20
1. Verfasser: Honda, Munechika (VerfasserIn)
Weitere Verfasser: Muramoto, Yasunori, Kuzuguchi, Tsuyoshi, Sawano, Sadanori, Machida, Masayuki, Koyama, Hiroyuki
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Comparative Study Journal Article DNA, Bacterial DNA, Plant T-DNA 4-Hydroxyphenylpyruvate Dioxygenase EC 1.13.11.27 Citrate (si)-Synthase EC 2.3.3.1