Use of aphid stylectomy and RT-PCR for the detection of transporter mRNAs in sieve elements
Unmodified samples of barley (Hordeum vulgare) sieve tube sap have been obtained by severing the stylets (stylectomy) of feeding aphids and collecting the exuding liquid. Primers were designed to direct the amplification of a series of specific cDNAs encoding barley proteins selected because of thei...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 53(2002), 369 vom: 31. Apr., Seite 631-7 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Aquaporins Cation Transport Proteins HAK1 protein, plant Membrane Transport Proteins Plant Proteins RNA, Messenger sucrose transport protein, plant Adenosine Triphosphatases |
| Zusammenfassung: | Unmodified samples of barley (Hordeum vulgare) sieve tube sap have been obtained by severing the stylets (stylectomy) of feeding aphids and collecting the exuding liquid. Primers were designed to direct the amplification of a series of specific cDNAs encoding barley proteins selected because of their significance in sieve tube function. mRNA encoding the H(+)/sucrose co-transporter SUT1, a putative aquaporin and the H(+)/ATPase PPA1 were detected in sieve tube sap. These mRNA species appear to be present at very low concentrations. mRNA encoding the potassium transporter HAK1 could not be detected. The results strongly suggest that some mRNA species are imported into sieve elements, which are enucleate, from neighbouring companion cells |
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| Beschreibung: | Date Completed 23.05.2002 Date Revised 27.04.2022 published: Print Citation Status MEDLINE |
| ISSN: | 1460-2431 |