Use of aphid stylectomy and RT-PCR for the detection of transporter mRNAs in sieve elements

Unmodified samples of barley (Hordeum vulgare) sieve tube sap have been obtained by severing the stylets (stylectomy) of feeding aphids and collecting the exuding liquid. Primers were designed to direct the amplification of a series of specific cDNAs encoding barley proteins selected because of thei...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 53(2002), 369 vom: 31. Apr., Seite 631-7
1. Verfasser: Doering-Saad, Christian (VerfasserIn)
Weitere Verfasser: Newbury, H John, Bale, Jeff S, Pritchard, Jeremy
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Aquaporins Cation Transport Proteins HAK1 protein, plant Membrane Transport Proteins Plant Proteins RNA, Messenger sucrose transport protein, plant Adenosine Triphosphatases EC 3.6.1.-