Thermal effects on reverse transcription : improvement of accuracy and processivity in cDNA synthesis
Reverse transcription, coupled with DNA amplification, has been widely used for molecular analysis of RNAs. Reverse transcriptases are retroviral DNA polymerases that can synthesize DNA from both RNA and DNA. In general, because of the lack of 3'-->5' exonuclease activity in retroviral...
| Publié dans: | BioTechniques. - 1991. - 30(2001), 5 vom: 01. Mai, Seite 1074-8, 1080, 1082, passim |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. DNA, Complementary Deoxyribonucleotides Gene Products, pol RNA 63231-63-0 RNA-Directed DNA Polymerase EC 2.7.7.49 |