Thermal effects on reverse transcription improvement of accuracy and processivity in cDNA synthesis

Thermal effects on reverse transcription : improvement of accuracy and processivity in cDNA synthesis

Reverse transcription, coupled with DNA amplification, has been widely used for molecular analysis of RNAs. Reverse transcriptases are retroviral DNA polymerases that can synthesize DNA from both RNA and DNA. In general, because of the lack of 3'-->5' exonuclease activity in retroviral...

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Veröffentlicht in:BioTechniques. - 1993. - 30(2001), 5 vom: 01. Mai, Seite 1074-8, 1080, 1082, passim
1. Verfasser: Malboeuf, C M (VerfasserIn)
Weitere Verfasser: Isaacs, S J, Tran, N H, Kim, B
Format: Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, U.S. Gov't, P.H.S. DNA, Complementary Deoxyribonucleotides Gene Products, pol RNA 63231-63-0 RNA-Directed DNA Polymerase EC 2.7.7.49