Selection of scFv phages on intact cells under low pH conditions leads to a significant loss of insert-free phages
Display of functional antibody fragments on the surface of filamentous bacteriophages allows fast selection of specific phage antibodies against a variety of target antigens. However, enrichment of single chain variable fragment (scFv)-displaying phages is often hampered by the abundance of bacterio...
| Publié dans: | BioTechniques. - 1991. - 30(2001), 2 vom: 09. Feb., Seite 404-8, 410, 412-3 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Immunoglobulin Fragments Peptide Library immunoglobulin Fv |