Selection of scFv phages on intact cells under low pH conditions leads to a significant loss of insert-free phages
Display of functional antibody fragments on the surface of filamentous bacteriophages allows fast selection of specific phage antibodies against a variety of target antigens. However, enrichment of single chain variable fragment (scFv)-displaying phages is often hampered by the abundance of bacterio...
| Veröffentlicht in: | BioTechniques. - 1991. - 30(2001), 2 vom: 09. Feb., Seite 404-8, 410, 412-3 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2001
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Immunoglobulin Fragments Peptide Library immunoglobulin Fv |