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01000caa a22002652 4500 |
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NLM110938496 |
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DE-627 |
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20240114232358.0 |
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tu |
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231222s2001 xx ||||| 00| ||eng c |
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|a pubmed24n1253.xml
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|a (DE-627)NLM110938496
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|a (NLM)11181713
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Row, P E
|e verfasserin
|4 aut
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| 245 |
1 |
4 |
|a The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
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| 264 |
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1 |
|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 02.08.2001
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| 500 |
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|a Date Revised 09.01.2024
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to import or form early import intermediates with precursor proteins. Incubation of chloroplasts with N-ethyl maleimide, diethyl pyrocarbonate, phenylglyoxal, 4,4'-di-isothiocyanatostilbene 2,2'-disulphonic acid (DIDS), dicyclohexylcarbodiimide (DCCD), and 1-ethyl- 3-dimethylaminopropylcarbodiimide (EDC) inhibited both import and formation of early import intermediates with precursor proteins by chloroplasts. This suggests that one or more of the binding components of the chloroplast protein import machinery contains functionally important solvent-exposed cysteine, histidine, arginine, and aspartate/glutamate residues, as well as functionally important lysine and aspartate/ glutamate residues in a hydrophobic environment
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Amino Acids
|2 NLM
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| 650 |
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7 |
|a Chloroplast Proteins
|2 NLM
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| 650 |
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7 |
|a Cross-Linking Reagents
|2 NLM
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| 650 |
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7 |
|a Enzyme Inhibitors
|2 NLM
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| 650 |
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7 |
|a Membrane Proteins
|2 NLM
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| 650 |
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7 |
|a Membrane Transport Proteins
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Protein Precursors
|2 NLM
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| 650 |
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7 |
|a import intermediate associated protein 100
|2 NLM
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| 650 |
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7 |
|a 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
|2 NLM
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| 650 |
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7 |
|a 27816-59-7
|2 NLM
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| 650 |
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7 |
|a Dicyclohexylcarbodiimide
|2 NLM
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| 650 |
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7 |
|a 538-75-0
|2 NLM
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| 650 |
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7 |
|a 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide
|2 NLM
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| 650 |
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7 |
|a 73U5123RNT
|2 NLM
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| 650 |
|
7 |
|a Ferredoxin-NADP Reductase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.18.1.2
|2 NLM
|
| 650 |
|
7 |
|a Ribulose-Bisphosphate Carboxylase
|2 NLM
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| 650 |
|
7 |
|a EC 4.1.1.39
|2 NLM
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| 650 |
|
7 |
|a Diethyl Pyrocarbonate
|2 NLM
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| 650 |
|
7 |
|a LMR3LZG146
|2 NLM
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| 650 |
|
7 |
|a Phenylglyoxal
|2 NLM
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| 650 |
|
7 |
|a N45G3015PA
|2 NLM
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| 650 |
|
7 |
|a Ethylmaleimide
|2 NLM
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| 650 |
|
7 |
|a O3C74ACM9V
|2 NLM
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| 650 |
|
7 |
|a Ethyldimethylaminopropyl Carbodiimide
|2 NLM
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| 650 |
|
7 |
|a RJ5OZG6I4A
|2 NLM
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| 700 |
1 |
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|a Gray, J C
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 52(2001), 354 vom: 05. Jan., Seite 57-66
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:52
|g year:2001
|g number:354
|g day:05
|g month:01
|g pages:57-66
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 52
|j 2001
|e 354
|b 05
|c 01
|h 57-66
|