The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates

In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to import or form early import intermediates with precursor proteins. Incubation of chloroplasts with N-ethy...

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 52(2001), 354 vom: 05. Jan., Seite 57-66
1. Verfasser:	Row, P E (VerfasserIn)
Weitere Verfasser:	Gray, J C
Format:	Aufsatz
Sprache:	English
Veröffentlicht:	2001
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Amino Acids Chloroplast Proteins Cross-Linking Reagents Enzyme Inhibitors Membrane Proteins Membrane Transport Proteins Plant Proteins Protein Precursors mehr... import intermediate associated protein 100 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 27816-59-7 Dicyclohexylcarbodiimide 538-75-0 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide 73U5123RNT Ferredoxin-NADP Reductase EC 1.18.1.2 Ribulose-Bisphosphate Carboxylase EC 4.1.1.39 Diethyl Pyrocarbonate LMR3LZG146 Phenylglyoxal N45G3015PA Ethylmaleimide O3C74ACM9V Ethyldimethylaminopropyl Carbodiimide RJ5OZG6I4A

Beschreibung
Zusammenfassung:	In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to import or form early import intermediates with precursor proteins. Incubation of chloroplasts with N-ethyl maleimide, diethyl pyrocarbonate, phenylglyoxal, 4,4'-di-isothiocyanatostilbene 2,2'-disulphonic acid (DIDS), dicyclohexylcarbodiimide (DCCD), and 1-ethyl- 3-dimethylaminopropylcarbodiimide (EDC) inhibited both import and formation of early import intermediates with precursor proteins by chloroplasts. This suggests that one or more of the binding components of the chloroplast protein import machinery contains functionally important solvent-exposed cysteine, histidine, arginine, and aspartate/glutamate residues, as well as functionally important lysine and aspartate/ glutamate residues in a hydrophobic environment
Beschreibung:	Date Completed 02.08.2001 Date Revised 09.01.2024 published: Print Citation Status MEDLINE
ISSN:	1460-2431