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|a eng
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|a Patrone, G
|e verfasserin
|4 aut
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|a Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR
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|c 2000
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|a Date Completed 01.03.2001
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|a Date Revised 29.01.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a In this report, we present a fluorescence-based approach to the assessment of cellular gene expression and transcription rates. Nuclear run-on was performed by supplying biotin-16-UTP to nuclei, and labeled transcripts were bound to streptavidin-coated magnetic beads. Total cDNA was then synthesized by means of random hexamer primed reverse transcription of captured molecules. To monitor transcript abundance in cDNA, both from nuclear run-on and total RNA, we propose a semiquantitative PCR approach based on the use of fluorescent primers
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| 700 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 29(2000), 5 vom: 01. Nov., Seite 1012-4, 1016-7
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|x 0736-6205
|7 nnns
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| 773 |
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